What this calculator does
Ligation molar ratio (insert:vector) works out insert mass needed for a target insert-to-vector molar ratio in a DNA cloning ligation. Enter your own figures above and the answer updates as you type: nothing is fixed in the code, so the result reflects exactly the numbers you supply.
The formula this calculator evaluates is printed under the tool and explained below, so you can check the working by hand or reuse it in a spreadsheet.
The formula
The inputs explained
| Field | What to enter |
|---|---|
| Vector mass used (ng) | A number, measured in ng. Starts at 50. |
| Vector length (kb) | A number, measured in kb. Starts at 3. |
| Insert length (kb) | A number, measured in kb. Starts at 1. |
| Target molar ratio (insert:vector) | A number. Starts at 3. |
Worked examples
Every figure in the tables below is produced by this page’s own calculator at build time, so the numbers and the tool always agree. Select any row to load that scenario.
How the answer changes with vector mass used
Every other input is held at the calculator’s starting values while vector mass used varies. Select any row to load that scenario into the calculator.